RNA Methods: Northern blot

Ribosomal RNA Detection on Northern Blot Employing a Specific Oligonucleotide

reconstituted basement membrane matrix is not correlated to the malignant metastatic cell phenotype. Cancer Res. 51:405-414. 4.Price, J.T. and E.W. Thompson. 1999. Models for studying cellular invasion of basement membranes. Methods Mol. Biol. 129:231-249. 5.Rodriguez, G.C., C. Haisley, J. Hurteau, T.L. Moser, R. Whitaker, R.C. J. Bast, and M.S. Stack. 2001. Regulation of invasion of epithelial...

Evaluation of four viroid RNA extraction methods for the molecular diagnosis of CEVd in Citrus lemon using RT-PCR, Dot blot and Northern blot

An effi cient method for RNA extraction that leads to RNA high yield and purity is a technical issue relevant for development and optimization of molecular diagnostic methods aimed to detect viroid infections in citrus varieties. Residual contaminants may affect RNA detection depending on the molecular diagnosis approaches. This condition can be evaluated through RNA absorption spectrum analysi...

Enhanced Northern Blot detection of small RNA species in Drosophila melanogaster.

The last decades have witnessed the explosion of scientific interest around gene expression control mechanisms at the RNA level. This branch of molecular biology has been greatly fueled by the discovery of noncoding RNAs as major players in post-transcriptional regulation. Such a revolutionary perspective has been accompanied and triggered by the development of powerful technologies for profili...

Northern blot detection of endogenous small RNAs (∼14 nt) in bacterial total RNA extracts

Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as approximately 14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5'-digoxigenin-endlabeled for non-radioactive probe detection with 1-ethyl-3-(3-dimethylaminopropyl)...

18S ribosomal RNA detection on Northern blot employing a specific oligonucleotide.